ULK3 Antibody

• Background:

  ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1) . Knockout of ULK genes results in a severe defect in the autophagy pathway (2) . ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3) . ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4) . Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5) .

• NCBI Gene ID:

  25989

• Swiss Prot:

  Q6PHR2

• Accession Number:

  NP_001092906

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Autophagy

• Purification:

  ULK3 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1303 - Human Brain Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat: (95%), Mouse: (89%), Chicken: (79%)

• Buffer:

  ULK3 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  ULK3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.

• Symbol:

  ULK3

• Positive Control 2:

  Cat. No. 10-301 - Human Brain Tissue Slide

• NCBI Official Name:

  Unc-51 like kinase 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209–18.

• Background Reference 02:

  Lee EJ and Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy 2011; 7:689-95.

• Background Reference 03:

  Zhou X, Babu JR, da Silva S, et al. Unc-51-like kinase 1/2-mediated endocytic processes regulate filopodia extension and branching of sensory axons. Proc. Natl. Acad. Sci. USA 2007; 104:5842-7.

• Background Reference 04:

  Maloverjan A, Piirsoo M, Michelson P, et al. Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway. Exp. Cell Res. 2010; 316:627-37.

• Other Product Names:

  ULK3 Antibody: Unc-51-like kinase 3

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  150456432

• Physical Properties:

  Liquid