B-raf Antibody

• Background:

  B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

• NCBI Gene ID:

  673

• Swiss Prot:

  P15056

• Accession Number:

  P15056

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  7379

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain. B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines5777 7379

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  B-raf Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1303 - Human Brain Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (100%)

• Buffer:

  B-raf Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  B-raf antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  This antibody will not cross-react with C-raf.

• Isoform:

  Human B-raf has one isoform (766aa, 84kD) . Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD) . Rat B-raf a has one isoform (712aa, 80kD) . 7379 can detect in human B-raf and mouse isoform 1.

• Symbol:

  BRAF

• Positive Control 2:

  Cat. No. 11-801 - Human Small Intestine Tissue Slide

• Positive Control 3:

  Cat. No. 1224 – Daudi Cell Lysate

• Positive Control 4:

  Cat. No. 1204 – K562 Cell Lysate

• NCBI Official Name:

  V-raf murine sarcoma viral oncogene homolog B1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  McCubrey et al. Biophys. Acta 2007; 1773:1263-84.

• Background Reference 02:

  Madhunapantula and Robertsonet al. Cancer Res.2008; 68:5-8.

• Background Reference 03:

  Sarkozy et al. Hum. Mutat.2009; 30:695-702.

• Other Product Names:

  B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  50403720

• Physical Properties:

  Liquid