PRICKLE2 Antibody

• Background:

  PRICKLE2 Antibody: PRICKLE2, also known as Pk2 or EPM5, is a member of a highly conserved protein family that function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity. Both PRICKLE2 and the related protein PRICKLE1 are expressed in postmitotic neurons and promote neurite outgrowth, and both proteins promote neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. PRICKLE2 localizes to the postsynaptic density and interacts with PSD-95 and NMDA receptors. Defects in the gene encoding PRICKLE2 are associated with autosomal recessive progressive myoclonic epilepsy.

• NCBI Gene ID:

  166336

• Swiss Prot:

  Q7Z3G6

• Accession Number:

  NP_942559

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  PRICKLE2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1288 - A20 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PRICKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PRICKLE2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  Two isoforms of PRICKLE2 are known to exist. PRICKLE2 antibody is predicted to not cross-react with other PRICKLE protein family members.

• Symbol:

  PRICKLE2

• NCBI Official Name:

  Prickle homolog 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56.

• Background Reference 02:

  Veeman MT, Slusarski DC, Kaykas A, et al. Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Curr. Biol. 2003; 13:680-5.

• Background Reference 03:

  Okuda H, Miyata S, Mori Y, et al. Mouse Prickle1 and Prickle2 are expressed in postmitotic neurons and promote neurite outgrowth. FEBS Lett. 2007; 581:4754-60

• Background Reference 04:

  Fujimura L, Watanabe-Takano H, Sato Y, et al. Prickle promotes neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. Neurosci. Lett. 2009; 467:6-10.

• Other Product Names:

  PRICKLE2 Antibody: EPM5, Prickle-like protein 2

• Tested Applications:

  ELISA, WB, IF

• Protein ID:

  38524620

• Physical Properties:

  Liquid