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B-raf Antibody

CAT: 0223-5777-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-5777-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
NCBI Gene ID
673
Swiss Prot
P15056
Accession Number
P15056
Host
Chicken
Reactivity
Human, Mouse, Rat
Immunogen
5777
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain. B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines5777 7379
Field of Research
Cancer, Cell Cycle
Purification
B-raf Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1303 - Human Brain Tissue Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Chicken: (93%)
Buffer
B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
B-raf antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgY
Isoform
Human B-raf has one isoform (766aa, 84kD) . Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD) . Rat B-raf has one isoform (712aa, 80kD) . 5777 can detect in human B-raf and mouse isoform 1.
Symbol
BRAF
Positive Control 2
Cat. No. 1210 – 293 Cell Lysate
Positive Control 3
Cat. No. 1224 – Daudi Cell Lysate
Positive Control 4
Cat. No. 1204 – K562 Cell Lysate
NCBI Official Name
V-raf murine sarcoma viral oncogene homolog B1
NCBI Organism
Homo sapiens
Background Reference 01
McCubrey et al. Biophys. Acta 2007; 1773:1263-84.
Background Reference 02
Madhunapantula and Robertsonet al. Cancer Res.2008; 68:5-8.
Background Reference 03
Sarkozy et al. Hum. Mutat.2009; 30:695-702.
Other Product Names
B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf
Tested Applications
ELISA, WB
Protein ID
50403720
Physical Properties
Liquid

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