AFAP1L2 Antibody

• Background:

  AFAP1L2 Antibody: AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.

• NCBI Gene ID:

  84632

• Swiss Prot:

  Q8N4X5

• Accession Number:

  NP_001001936

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  AFAP1L2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1404 - Mouse Liver Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%)

• Buffer:

  AFAP1L2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  AFAP1L2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  AFAP1L2

• Positive Control 2:

  Cat. No. 10-201 - Human Liver Tissue Slide

• NCBI Official Name:

  Actin filament associated protein 1-like 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Xu J, Bai X-H, Lodyga M, et al. XB130, a novel adaptor protein for signal transduction. J. Biol. Chem.2007; 282:16401-12.

• Background Reference 02:

  Lodyga M, De Falco V, Bai XH, et al. XB130, a tissue-specific adaptor protein that couples the RET/PTC oncogenic kinase to PI 3-kinase pathway. Oncogene2009; 28:937-49.

• Other Product Names:

  AFAP1L2 Antibody: XB130, KIAA1914, CTB-1144G6.4, XB130, Actin filament-associated protein 1-like 2, AFAP1-like protein 2

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  50897850

• Physical Properties:

  Liquid