TBC1D4 Antibody

• Background:

  TBC1D4 Antibody: TBC1D4, also known as the Akt substrate AS160, was initially identified as an Akt substrate containing a GTPase-activating domain that regulates GLUT4 trafficking, with activation following insulin stimulation. TBC1D4 truncations in humans is a major cause of dominant inherited insulin resistance. The loss of TBC1D4 results in the accumulation of GLUT4 in compartments that are primed for fusion in basal adipocytes.

• NCBI Gene ID:

  9882

• Swiss Prot:

  O60343

• Accession Number:

  NP_055647

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  TBC1D4 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 10-635 - Human Prostate Tissue Slide

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (89%)

• Buffer:

  TBC1D4 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TBC1D4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Symbol:

  TBC1D4

• NCBI Official Name:

  TBC1 domain family, member 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kane S, Sano H, Liu SCH, et al. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain. J. Biol. Chem. 2002; 277:22115-8.

• Background Reference 02:

  Sano H, Kane S, Sano E, et al. Insulin-stimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation. J. Biol. Chem. 2003; 278:14599-602.

• Background Reference 03:

  Dash S, Sano H, Rochford JJ, et al. A truncation mutation in TBC1D4 in a family with acanthosis nigricans and postprandial hyperinsulinemia. Proc. Natl. Acad. Sci. U.S.A. 2009; 106:9350–5.

• Background Reference 04:

  Brewer PD, Romenskaia I, Kanow MA, et al. Loss of AS160 Akt substrate causes Glut4 protein to accumulate in compartments that are primed for fusion in basal adipocytes. J. Biol. Chem. 2011; 286:26287-97.

• Other Product Names:

  TBC1D4 Antibody: AS160, AS160, KIAA0603, TBC1 domain family member 4, Akt substrate of 160 kDa

• Tested Applications:

  ELISA, IHC-P

• Protein ID:

  114688046

• Physical Properties:

  Liquid