TEM1 Antibody

• Background:

  TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.

• NCBI Gene ID:

  57124

• Swiss Prot:

  Q9HCU0

• Accession Number:

  NP_065137

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  4359

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar TEM-1 expression profile in human and mouse cell lines detected by two independent anti-TEM-1 antibodies that recognize different epitopes, 4357 against C-terminus domain and 4359 against N-terminus domain. TEM-1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4357 4359

• Field of Research:

  Homeostasis, Cancer

• Purification:

  TEM1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1320 - Human Colon Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse (100%) ; Rat: (93%)

• Buffer:

  TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TEM1 antibody can be stored at 4˚ C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least two isoforms of TEM1 are known to exist; this antibody recognizes only the larger isoform.

• Isoform:

  Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD), and isoform 2 (433aa, 46kD) . Mouse TEM-1 has one isoform (765aa, 82kD) and Rat TEM-1 also has one isoform (764aa, 82kD) . 4359 can detect human isoform 1, mouse and rat TEM-1.

• Symbol:

  CD248

• Positive Control 2:

  Cat. No. 1223 - Caco-2 Cell Lysate

• Positive Control 3:

  Cat. No. 1219 – MCF-7 Cell Lysate

• Positive Control 4:

  Cat. No. 1220 – SK-N-SH Cell Lysate

• NCBI Official Name:

  CD248 molecule, endosialin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rettig et al. Proc. Natl. Acad. Sci. USA 1992; 89:10832-6.

• Background Reference 02:

  Christian et al. J. Biol. Chem. 2001; 276:7408-14.

• Background Reference 03:

  Nanda et al. Curr. Opin. Oncol. 2004; 16:44-9.

• Background Reference 04:

  Nanda et al. Proc. Natl. Acad. Sci. USA 2006; 103:3351-6.

• Other Product Names:

  TEM1 Antibody: TEM1, CD164L1, TEM1, Endosialin, Tumor endothelial marker 1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  9966885

• Physical Properties:

  Liquid