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PD-L1 Antibody

CAT: 0223-4059-01Size: 0.02 mgDry Ice: NoHazardous: No
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Background
PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1) . The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3) . Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4) .
NCBI Gene ID
29126
Swiss Prot
Q9NZQ7
Accession Number
NP_054862
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
4059
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation (Figure 2) shows similar PD-L1 expression profile in both human and mouse cell lines detected by two independent anti-PD-L1 antibodies that recognize different epitopes, 4059 against the center of human PD-L1 and RF16035 against the extracellular domain. PD-L1 proteins are detected in most of the cell lines, but not in A549 and THP-1 cells by the two independent antibodies. Independent Antibody Validation4059 RF16035 siRNA Knockdown Validation (Figure 3) : Anti-PD-L1 antibody (4059) specificity was further verified by PD-L1 specific siRNA knockdown. PD-L1 signal in HeLa cells transfected with PD-L1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA Knockdown Validation4059 Overexpression Validation (Figure 4) : Anti-PD-L1 antibodies (4059) can detect the overexpression of PD-L1 protein in 293 cells transfected with PD-L1. Overexpression Validation4059
Field of Research
Apoptosis
Purification
PD-L1 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Rat: (77%), Mouse: (71%)
Buffer
PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
PD-L1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Specificity
PD-L1 antibody has no cross-reactivity to PD-L2.
Isoform
Human PD-L1 has 3 isoforms, including isoform 1 (290aa, 33.3kD), isoform 2 (176aa, 20.2kD), and isoform 3 (178aa, 20.5kD) . This antibody detects human isoform 1&3, mouse and rat PD-L1 (290aa, 33kD for both of them) .
Symbol
CD274
Positive Control 2
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 3
Cat. No. 1204 - K562 Cell Lysate
Positive Control 4
Cat. No. 10-501 - Human Heart Tissue Slide
Positive Control 5
Cat. No. 1301 - Human Heart Tissue Lysate
NCBI Official Name
CD274 molecule
NCBI Organism
Homo sapiens
Background Reference 01
Freeman et al. Exp. Med. 2000; 192:1027-34.
Background Reference 02
Burr et al. Nature 2017; 549:101-5.
Background Reference 03
Mezzadra et al. Nature 2017; 549:106-10.
Background Reference 04
Dong et al. Nat. Med. 1999 5:1365-9.
Citation 01
29416054
Citation 02
21523733
Citation 03
24955518
Citation 04
26483045
Citation 05
29024471
Citation 06
26351349
Citation 07
22461641
Citation 08
29704674
Citation 09
28751245
Citation 10
24866169
Citation 11
29034543
Citation 12
29610255
Citation 13
24130734
Citation 14
29309297
Citation 15
26175924
Citation 16
27799141
Citation 17
25672395
Citation 18
23606867
Citation 19
30059678
Citation 20
25937270
Other Product Names
PD-L1 Antibody: B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, B7 homolog 1
Tested Applications
ELISA, WB, IHC-P, IF, Flow
Protein ID
7661534
Physical Properties
Liquid

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