PD-L1 Antibody

• Background:

  PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1) . The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3) . Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4) .

• NCBI Gene ID:

  29126

• Swiss Prot:

  Q9NZQ7

• Accession Number:

  NP_054862

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  4059

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation (Figure 2) shows similar PD-L1 expression profile in both human and mouse cell lines detected by two independent anti-PD-L1 antibodies that recognize different epitopes, 4059 against the center of human PD-L1 and RF16035 against the extracellular domain. PD-L1 proteins are detected in most of the cell lines, but not in A549 and THP-1 cells by the two independent antibodies. Independent Antibody Validation4059 RF16035 siRNA Knockdown Validation (Figure 3) : Anti-PD-L1 antibody (4059) specificity was further verified by PD-L1 specific siRNA knockdown. PD-L1 signal in HeLa cells transfected with PD-L1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA Knockdown Validation4059 Overexpression Validation (Figure 4) : Anti-PD-L1 antibodies (4059) can detect the overexpression of PD-L1 protein in 293 cells transfected with PD-L1. Overexpression Validation4059

• Field of Research:

  Apoptosis

• Purification:

  PD-L1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat: (77%), Mouse: (71%)

• Buffer:

  PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PD-L1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  PD-L1 antibody has no cross-reactivity to PD-L2.

• Isoform:

  Human PD-L1 has 3 isoforms, including isoform 1 (290aa, 33.3kD), isoform 2 (176aa, 20.2kD), and isoform 3 (178aa, 20.5kD) . This antibody detects human isoform 1&3, mouse and rat PD-L1 (290aa, 33kD for both of them) .

• Symbol:

  CD274

• Positive Control 2:

  Cat. No. 1224 - Daudi Cell Lysate

• Positive Control 3:

  Cat. No. 1204 - K562 Cell Lysate

• Positive Control 4:

  Cat. No. 10-501 - Human Heart Tissue Slide

• Positive Control 5:

  Cat. No. 1301 - Human Heart Tissue Lysate

• NCBI Official Name:

  CD274 molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Freeman et al. Exp. Med. 2000; 192:1027-34.

• Background Reference 02:

  Burr et al. Nature 2017; 549:101-5.

• Background Reference 03:

  Mezzadra et al. Nature 2017; 549:106-10.

• Background Reference 04:

  Dong et al. Nat. Med. 1999 5:1365-9.

• Citation 01:

  29416054

• Citation 02:

  21523733

• Citation 03:

  24955518

• Citation 04:

  26483045

• Citation 05:

  29024471

• Citation 06:

  26351349

• Citation 07:

  22461641

• Citation 08:

  29704674

• Citation 09:

  28751245

• Citation 10:

  24866169

• Citation 11:

  29034543

• Citation 12:

  29610255

• Citation 13:

  24130734

• Citation 14:

  29309297

• Citation 15:

  26175924

• Citation 16:

  27799141

• Citation 17:

  25672395

• Citation 18:

  23606867

• Citation 19:

  30059678

• Citation 20:

  25937270

• Other Product Names:

  PD-L1 Antibody: B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, B7 homolog 1

• Tested Applications:

  ELISA, WB, IHC-P, IF, Flow

• Protein ID:

  7661534

• Physical Properties:

  Liquid