TIM-1 Antibody

• Background:

  TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

• NCBI Gene ID:

  26762

• Swiss Prot:

  Q96D42

• Accession Number:

  NP_036338

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  3811

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar TIM-1 expression profile in human cell lines detected by two independent anti-TIM-1 antibodies that recognize different epitopes, 3809 against N-terminus domain and 3811 against the central domain. TIM-1 proteins are detected in all the tested cell lines except MCF-7 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3809 3811 siRNA knockdown validation (Figure 3) : Anti-TIM-1 antibody (3811) specificity was further verified by TIM-1 specific siRNA knockdown. TIM-1 signal in HeLa cells transfected with TIM-1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA knockdown validation3811

• Field of Research:

  Immunology, Neuroscience, Signal Transduction, Growth Factors

• Purification:

  TIM-1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1202 - A431 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (50%), Rat: (43.7%)

• Buffer:

  TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TIM-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human TIM-1 has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD) . This antibody detects human isoform b precursor. Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD) . Rat TIM-1 has only one isoform identified so far (307aa, 34kD) .

• Symbol:

  HAVCR1

• Positive Control 2:

  Cat. No. 1203 - A549 Cell Lysate

• Positive Control 3:

  Cat. No. 1224 - Daudi Cell Lysate

• Positive Control 4:

  Cat. No. 1210 - 293 Cell Lysate

• NCBI Official Name:

  Hepatitis A virus cellular receptor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Feigelstock et al. J. Virol. 1998; 72:6621-8

• Background Reference 02:

  Ichimura et al. J. Biol. Chem. 1998; 273:4135-42

• Background Reference 03:

  Meyers et al. Mol. Med. 2005; 11:362-9

• Background Reference 04:

  Mayers et al. Nat. Immunol. 2005;6:455-64.

• Other Product Names:

  TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1

• Tested Applications:

  ELISA, WB

• Protein ID:

  153085427

• Physical Properties:

  Liquid