APOBEC3G Antibody

• Background:

  APOBEC3G Antibody: The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5α which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.

• NCBI Gene ID:

  60489

• Swiss Prot:

  Q9HC16

• Accession Number:

  NP_068594

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Infectious Disease

• Purification:

  APOBEC3G Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1223 - Caco-2 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  APOBEC3G Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  APOBEC3G antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  APOBEC3G antibody will also detect the APOBEC3F isoform.

• Symbol:

  APOBEC3G

• Positive Control 2:

  Cat. No. 1306 - Human Spleen Tissue Lysate

• Positive Control 3:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• NCBI Official Name:

  Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.

• Background Reference 02:

  Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.

• Background Reference 03:

  Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.

• Background Reference 04:

  Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.

• Other Product Names:

  APOBEC3G Antibody: A3G, ARCD, ARP9, ARP-9, CEM15, CEM-15, MDS019, bK150C2.7, dJ494G10.1APOBEC-related cytidine deaminase, APOBEC-related protein

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  13399304

• Physical Properties:

  Liquid