TRIM5 alpha Antibody

• Background:

  TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.

• NCBI Gene ID:

  574288

• Swiss Prot:

  Q0PF16

• Accession Number:

  ABG67967

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Infectious Disease

• Purification:

  TRIM5 alpha Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1319 - Human Stomach Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TRIM5 alpha antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  TRIM5 alpha antibody will not cross-react with other isoforms of TRIM5.

• Symbol:

  TRIM5

• Positive Control 2:

  Cat. No. 1317 - Human Uterus Tissue Lysate

• Positive Control 3:

  Cat. No. 1314 - Human Thymus Tissue Lysate

• Positive Control 4:

  Cat. No. 11-401 - Human Uterus Tissue Slide

• NCBI Official Name:

  Tripartite motif-containing 5

• NCBI Organism:

  Macaca mulatta

• Background Reference 01:

  Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.

• Background Reference 02:

  Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.

• Background Reference 03:

  Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5α . Proc. Nat'l. Acad. Sci. USA 2004; 101:10774-9

• Other Product Names:

  TRIM5 alpha Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  110339457

• Physical Properties:

  Liquid