IL-21 Antibody

• Background:

  IL-21 Antibody: A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

• NCBI Gene ID:

  59067

• Swiss Prot:

  Q9HBE4

• Accession Number:

  NP_068575

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  2465

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell Lines (Figure 2) shows similar IL-21 expression profile in human and mouse cell lines detected by two independent anti-IL-21 antibodies that recognize different epitopes, 2465 against central domain and 2463 against C-terminus domain. IL-21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell Lines2465 2463 Recombinant Protein Test (Figure 4) : Anit-IL-21 antibodies (2465) detected human IL-21 recombinant protein. Recombinant Protein Test2465 Regulated expression validation (Figure 6, 7, 8) : IL-21 expression detected by anit-IL-21 antibodies (2465) was up down-regulated by WRT treatment (figure 7) or was up-regulated in Hp positive patients (figure 8) and in patients with Crohn’s disease (figure 6) . Regulated expression validation2465

• Field of Research:

  Chemokines & Cytokines, Cancer

• Purification:

  IL-21 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1209 - HL60 Cell Lysate

• Concentration:

  1 mg/ml

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (87%)

• Buffer:

  IL-21 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  IL-21 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.

• Isoform:

  Human IL-21 has 2 isoforms, including isoform 1 (162aa, 18.7kD) and isoform 2 (153aa, 17.6kD) . Mouse IL-21 has one isoform (146aa, 16.8kD) and Rat IL-21 also has one isoform (146aa, 17.1kD) . 2465 can detect both human isoforms and also detect mouse and rat.

• Symbol:

  IL21

• Positive Control 2:

  Cat. No. 17-004 - K-562 Cell Slide

• NCBI Official Name:

  Interleukin 21

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Parrish-Novak et al. Nature 2000; 408:57-63.

• Background Reference 02:

  Ozaki et al. Proc. Natl. Acad. Sci. USA. 2000; 97:11439-44.

• Background Reference 03:

  Asao et al. J. Immunol. 2001; 167:1-5.

• Citation 01:

  15765404

• Citation 02:

  22785229

• Citation 03:

  17442980

• Other Product Names:

  IL-21 Antibody: Za11, IL-21, Interleukin-21, Za11

• Tested Applications:

  ELISA, WB, ICC

• Protein ID:

  11141875

• Physical Properties:

  Liquid