PERP Antibody

• Background:

  PERP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.

• NCBI Gene ID:

  64058

• Swiss Prot:

  Q9JK95

• Accession Number:

  NP_071404

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  2451

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 1) shows similar PERP expression profile in human cell lines detected by two independent anti-PERP antibodies that recognize different epitopes, 2451 against C-terminus domain and 57-777 against C-terminus domain. PERP proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2451 57-777

• Field of Research:

  Apoptosis

• Purification:

  PERP Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1202 - A431 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat (100%) ; Mouse (82%)

• Buffer:

  PERP Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PERP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human PERP has one isoform (193aa, 21kD) . Mouse PERP has one isoform (193aa, 21.6kD) and Rat PERP also has one isoform (193aa, 21.6kD) . 2451 can detect human, mouse and rat.

• Symbol:

  Perp

• Positive Control 2:

  Cat. No. 17-002 - A-431 Cell Slide

• NCBI Official Name:

  PERP, TP53 apoptosis effector

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Matsuoka et al. Science 1998; 282:1893-7.

• Background Reference 02:

  Tanaka et al. Nature 2000; 404:42-9.

• Background Reference 03:

  Oda et al. Science 2000; 288:1053-8.

• Background Reference 04:

  Oda et al. Cell 2000; 102:849-62.

• Other Product Names:

  PERP Antibody: THW, KCP1, PIGPC1, KRTCAP1, 1110017A08Rik, Krtcap1, p53 apoptosis effector related to PMP-22, Keratinocyte-associated protein 1, KCP-1

• Tested Applications:

  ELISA, WB, ICC

• Protein ID:

  11528520

• Physical Properties:

  Liquid