IKK alpha Antibody

• Background:

  IKK alpha Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κ B is associated with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. The long-sought Iκ B kinase (IKK), which phosphorylates Iκ B, and mediates Iκ B degradation and NF-κ B activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ ) . IKKα and IKKβ interact with each other and both are essential for the NF-κ B activation. IKKα specifically phosphorylates IkB-alpha. IKKα is expressed in variety of human tissues.

• NCBI Gene ID:

  1147

• Swiss Prot:

  O15111

• Accession Number:

  AF009225

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Overexpression validation (Figure 8) : ASC expression detected by anit-ASC antibodies (2287) was shown in ASC transfected 293T cells, but not in WT 293T cells. Overexpression validation2287

• Field of Research:

  Signal Transduction

• Purification:

  IKK alpha Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/ml

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (94%), Mouse: (79%)

• Buffer:

  IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  IKK alpha antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  85 kDa

• Fragment:

  IgG

• Specificity:

  Antibody has no cross response to IKKb or IKKg.

• Symbol:

  CHUK

• Positive Control 2:

  Cat. No. 1205 - Jurkat Cell Lysate

• Positive Control 3:

  Cat. No. 17-005 - Jurkat Cell Slide

• NCBI Official Name:

  Conserved helix-loop-helix ubiquitous kinase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive Iκ B kinase that activates the transcription factor NF-κ B. Nature 1997;388:548-54

• Background Reference 02:

  Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an Iκ B kinase. Cell 1997;90:373-83

• Background Reference 03:

  Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The Iκ B kinase complex (IKK) contains two kinase subunits, IKKα and IKKβ , necessary for Iκ B phosphorylation and NF-κ B activation. Cell 1997;91:243-52

• Background Reference 04:

  Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. Iκ B kinase-β : NF-κ B activation and complex formation with Iκ B kinase-α and NIK. Science 1997;278:866-9

• Other Product Names:

  IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  2327068

• Physical Properties:

  Liquid