APEX2 (NM_014481) Human Recombinant Protein

• Background:

  Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

• Synonyms:

  APE2; APEXL2; XTH2; ZGRF2

• Gene ID:

  27301

• UniProt:

  Q9UBZ4, E5KN95

• Accession Number mRNA:

  NM_014481

• Chromosomal Location:

  Xp11.21

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Base excision repair

• Field of Research:

  Base excision repair

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  57.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  APEX2

• Species:

  Human

• Protein ID:

  NP_055296

• Overview:

  Recombinant protein of human APEX nuclease (apurinic/apyrimidinic endonuclease) 2 (APEX2), nuclear gene encoding mitochondrial protein

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/27301

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9UBZ4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_014481

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_055296