APEX2 Antibody (Center) [APR32630G]

• Background:

  Tissue Location: Highly expressed in brain and kidney. Weakly expressed in the fetal brain.

• Synonyms:

  DNA- (apurinic or apyrimidinic site) lyase 2, 31--, AP endonuclease XTH2, APEX nuclease 2, APEX nuclease-like 2, Apurinic-apyrimidinic endonuclease 2, AP endonuclease 2, APEX2, APE2, APEXL2, XTH2

• Gene Name:

  APEX2

• Gene ID:

  27301

• UniProt:

  Q9UBZ4

• Chromosomal Location:

  Nucleus. Cytoplasm. Mitochondrion. Note=Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents

• Applications:

  IHC-P, FC, IF, WB

• Dilution:

  WB--1:1000 IF--1:25 IHC-P--1:100~500 FC--1:10~50

• Form:

  Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Function:

  Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'- phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes.

• Molecular Weight:

  57401

• Storage Conditions:

  Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

• Overview:

  We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality APEX2 Antibody (Center) [APR32630G].

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/?term=27301

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9UBZ4