HSPA2 (NM_021979) Human Recombinant Protein

• Background:

  Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed:26865365) . Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  HSP70-2; HSP70-3

• Gene ID:

  3306

• UniProt:

  P54652, A0A024R6B5

• Accession Number mRNA:

  NM_021979

• Chromosomal Location:

  14q23.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome

• Field of Research:

  Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Stem cell - Pluripotency

• Molecular Weight:

  69.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  HSPA2

• Species:

  Human

• Protein ID:

  NP_068814

• Overview:

  Recombinant protein of human heat shock 70kDa protein 2 (HSPA2)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/3306

• Uniprot URL:

  https://www.uniprot.org/uniprot/P54652

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_021979

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_068814