SEMA4A (NM_022367) Human Recombinant Protein

• Background:

  This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF) -mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10) . Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

• Synonyms:

  CORD10; RP35; SEMAB; SEMB

• Gene ID:

  64218

• UniProt:

  Q9H3S1

• Accession Number mRNA:

  NM_022367

• Chromosomal Location:

  1q22

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Axon guidance

• Field of Research:

  Axon guidance

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Transmembrane

• Molecular Weight:

  83.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SEMA4A

• Species:

  Human

• Protein ID:

  NP_071762

• Overview:

  Recombinant protein of human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A (SEMA4A)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/64218

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9H3S1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_022367

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_071762