Cullin 2 (CUL2) (NM_003591) Human Recombinant Protein

• Background:

  Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346) . May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS (VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  MGC131970

• Gene ID:

  8453

• UniProt:

  Q13617, A0A140VKB1

• Accession Number mRNA:

  NM_003591

• Chromosomal Location:

  10p11.21

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis

• Field of Research:

  Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  86.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CUL2

• Species:

  Human

• Protein ID:

  NP_003582

• Overview:

  Recombinant protein of human cullin 2 (CUL2)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/8453

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q13617

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_003591

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_003582