Cul2 (BC026779) Mouse Recombinant Protein

• Background:

  Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (By similarity) . May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity) . The functional specificity of the ECS complex depends on the substrate recognition component. ECS (VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  1300003D18Rik; 4932411N15Rik; AI327301; mKIAA4106

• Gene ID:

  71745

• UniProt:

  Q9D4H8

• Accession Number mRNA:

  BC026779

• Chromosomal Location:

  18 A1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  86.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Cul2

• Species:

  Mouse

• Overview:

  Purified recombinant protein of Mouse cullin 2 (cDNA clone MGC:25783 IMAGE:4018298), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/71745

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9D4H8

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/BC026779