MRPS22 (NM_020191) Human Recombinant Protein

• Background:

  Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq. [provided by RefSeq, Jul 2008]

• Synonyms:

  C3orf5; COXPD5; GIBT; GK002; MRP-S22; ODG7; RPMS22

• Gene ID:

  56945

• UniProt:

  P82650

• Accession Number mRNA:

  NM_020191

• Chromosomal Location:

  3q23

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  41.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MRPS22

• Species:

  Human

• Protein ID:

  NP_064576

• Overview:

  Recombinant protein of human mitochondrial ribosomal protein S22 (MRPS22), nuclear gene encoding mitochondrial protein

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/56945

• Uniprot URL:

  https://www.uniprot.org/uniprot/P82650

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_020191

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_064576