Alpha Actinin (ACTN1) (NM_001102) Human Recombinant Protein

• Background:

  Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

• Synonyms:

  BDPLT15

• Gene ID:

  87

• UniProt:

  P12814, A0A024R694

• Accession Number mRNA:

  NM_001102

• Chromosomal Location:

  14q22-q24

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction

• Field of Research:

  Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, ES Cell Differentiation/IPS

• Molecular Weight:

  102.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ACTN1

• Species:

  Human

• Protein ID:

  NP_001093

• Overview:

  Recombinant protein of human actinin, alpha 1 (ACTN1), transcript variant 2

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/87

• Uniprot URL:

  https://www.uniprot.org/uniprot/P12814

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001102

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001093