CCN4 (NM_003882) Human Recombinant Protein

• Background:

  This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]

• Synonyms:

  WISP1; WISP1-OT1; WISP1-UT1; WISP1c; WISP1i; WISP1tc

• Gene ID:

  8840

• UniProt:

  O95388

• Accession Number mRNA:

  NM_003882

• Chromosomal Location:

  8q24.22

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - Wnt Signaling pathway

• Molecular Weight:

  38 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CCN4

• Species:

  Human

• Protein ID:

  NP_003873

• Overview:

  Recombinant protein of human WNT1 inducible signaling pathway protein 1 (WISP1), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/8840

• Uniprot URL:

  https://www.uniprot.org/uniprot/O95388

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_003882

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_003873