A1CF (NM_138933) Human Recombinant Protein

• Background:

  Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

• Synonyms:

  ACF; ACF64; ACF65; APOBEC1CF; ASP

• Gene ID:

  29974

• UniProt:

  Q9NQ94, A0A024QZI9

• Accession Number mRNA:

  NM_138933

• Chromosomal Location:

  10q11.23

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  64.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  A1CF

• Species:

  Human

• Protein ID:

  NP_620311

• Overview:

  Recombinant protein of human APOBEC1 complementation factor (A1CF), transcript variant 3

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/29974

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9NQ94

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_138933

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_620311