KCC2 (SLC12A5) (NM_020708) Human Recombinant Protein

• Background:

  K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]

• Synonyms:

  DEE34; EIEE34; EIG14; hKCC2; KCC2

• Gene ID:

  57468

• UniProt:

  Q9H2X9

• Accession Number mRNA:

  NM_020708

• Chromosomal Location:

  20q13.12

• Expression System:

  HEK293

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Transmembrane

• Molecular Weight:

  123.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SLC12A5

• Species:

  Human

• Protein ID:

  NP_065759

• Overview:

  Purified recombinant protein of Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2, with C-terminal MYC/DDK tag, expressed in HEK293 cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/57468

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9H2X9

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_020708

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_065759