Chk1 (CHEK1) (NM_001114122) Human Recombinant Protein

• Background:

  The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

• Synonyms:

  CHK1

• Gene ID:

  1111

• UniProt:

  O14757, B4DT73

• Accession Number mRNA:

  NM_001114122

• Chromosomal Location:

  11q24.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Cell cycle, p53 signaling pathway

• Field of Research:

  Cell cycle, p53 signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Protein Kinase, Stem cell - Pluripotency

• Molecular Weight:

  54.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CHEK1

• Species:

  Human

• Protein ID:

  NP_001107594

• Overview:

  Purified recombinant protein of Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1), transcript variant 3

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/1111

• Uniprot URL:

  https://www.uniprot.org/uniprot/O14757

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001114122

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001107594