Glutathione Peroxidase 3 (GPX3) (NM_002084) Human Recombinant Protein

• Background:

  The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]

• Synonyms:

  GPx-P; GSHPx-3; GSHPx-P

• Gene ID:

  2878

• UniProt:

  P22352

• Accession Number mRNA:

  NM_002084

• Chromosomal Location:

  5q33.1

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Arachidonic acid metabolism, Glutathione metabolism

• Field of Research:

  Arachidonic acid metabolism, Glutathione metabolism

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris, 8M Urea, pH8.0.

• Function:

  Druggable Genome, Secreted Protein

• Molecular Weight:

  5.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  GPX3

• Species:

  Human

• Protein ID:

  NP_002075

• Overview:

  Purified recombinant protein of Human glutathione peroxidase 3 (plasma) (GPX3), Gln21-Tyr72, with N-terminal His tag, expressed in E.coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2878

• Uniprot URL:

  https://www.uniprot.org/uniprot/P22352

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_002084

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_002075