H2az1 (NM_016750) Mouse Recombinant Protein

• Background:

  Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]

• Synonyms:

  H2A.; H2A.Z; H2a.z-1; H2A.Z1; H2af; H2afz

• Gene ID:

  51788

• UniProt:

  P0C0S6

• Accession Number mRNA:

  NM_016750

• Chromosomal Location:

  3 G3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  13.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  H2az1

• Species:

  Mouse

• Protein ID:

  NP_058030

• Overview:

  Purified recombinant protein of Mouse H2A histone family, member Z (H2afz), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/51788

• Uniprot URL:

  https://www.uniprot.org/uniprot/P0C0S6

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016750

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_058030