Eif2s1 (NM_026114) Mouse Recombinant Protein

• Background:

  Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  35kDa; 0910001O23Rik; 2410026C18Rik; Eif2a; eIF2alpha

• Gene ID:

  13665

• UniProt:

  Q6ZWX6

• Accession Number mRNA:

  NM_026114

• Chromosomal Location:

  12 C3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  36.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Eif2s1

• Species:

  Mouse

• Protein ID:

  NP_080390

• Overview:

  Purified recombinant protein of Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/13665

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q6ZWX6

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_026114

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_080390