Ndn (NM_010882) Mouse Recombinant Protein

• Background:

  Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI528698; Peg6

• Gene ID:

  17984

• UniProt:

  P25233

• Accession Number mRNA:

  NM_010882

• Chromosomal Location:

  7 C

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  37.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ndn

• Species:

  Mouse

• Protein ID:

  NP_035012

• Overview:

  Purified recombinant protein of Mouse necdin (Ndn), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/17984

• Uniprot URL:

  https://www.uniprot.org/uniprot/P25233

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_010882

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_035012