Hmgcl (NM_008254) Mouse Recombinant Protein

• Background:

  Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S) -3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AW476067; HL

• Gene ID:

  15356

• UniProt:

  P38060

• Accession Number mRNA:

  NM_008254

• Chromosomal Location:

  4 68.14 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  34.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Hmgcl

• Species:

  Mouse

• Protein ID:

  NP_032280

• Overview:

  Purified recombinant protein of Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/15356

• Uniprot URL:

  https://www.uniprot.org/uniprot/P38060

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008254

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032280