Xrcc4 (NM_028012) Mouse Recombinant Protein

• Background:

  Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. Binds to DNA and to DNA ligase IV (LIG4) . The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2310057B22Rik; AW413319; AW545101

• Gene ID:

  108138

• UniProt:

  Q924T3, A0A0R4J024

• Accession Number mRNA:

  NM_028012

• Chromosomal Location:

  13 C3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  37.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Xrcc4

• Species:

  Mouse

• Protein ID:

  NP_082288

• Overview:

  Purified recombinant protein of Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/108138

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q924T3

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_028012

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_082288