Galt (NM_016658) Mouse Recombinant Protein

• Background:

  The protein encoded by this gene is the second enzyme in the Leloir pathway, the metabolic pathway for D-galactose catabolism. It catalyzes the conversion of galactose-1-phosphate and uridine diphosphate-glucose to glucose-1-phosphate and uridine diphosphate galactose. Deficiency of this enzyme causes the genetic metabolic disorder galactosemia. Mice lacking this protein accumulate high levels of galactose and galactose-1 phosphate but are viable and fertile. This protein is negatively regulated through signaling by the polypeptide hormone prolactin, specifically via the short isoform of the prolactin receptor and the transcription factor Forkhead box O3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

• Synonyms:

  AW553376

• Gene ID:

  14430

• UniProt:

  Q03249, A2AMS3

• Accession Number mRNA:

  NM_016658

• Chromosomal Location:

  4 22.07 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  41.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Galt

• Species:

  Mouse

• Protein ID:

  NP_057867

• Overview:

  Purified recombinant protein of Mouse galactose-1-phosphate uridyl transferase (Galt), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/14430

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q03249

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016658

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_057867