Rad9a (NM_011237) Mouse Recombinant Protein

• Background:

  Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) . The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Rad9

• Gene ID:

  19367

• UniProt:

  Q9Z0F6

• Accession Number mRNA:

  NM_011237

• Chromosomal Location:

  19 A

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  42.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Rad9a

• Species:

  Mouse

• Protein ID:

  NP_035367

• Overview:

  Purified recombinant protein of Mouse RAD9 checkpoint clamp component A (Rad9a), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/19367

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9Z0F6

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_011237

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_035367