Wdr4 (BC039272) Mouse Recombinant Protein

• Background:

  Non-catalytic component of a methyltransferase complex required for the formation of N (7) -methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320) . In the methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit (By similarity) . Required for the formation of N (7) -methylguanine at position 46 (m7G46) in tRNA (PubMed:29983320) . Also required for the formation of N (7) -methylguanine at internal sites in a subset of mRNAs (By similarity) . Also required for methylation of a specific subset of miRNAs, such as let-7 (By similarity) . Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320) . Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed:29574139) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI415180; AI448349; D530049K22Rik

• Gene ID:

  57773

• UniProt:

  Q9EP82

• Accession Number mRNA:

  BC039272

• Chromosomal Location:

  17 B1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  45.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Wdr4

• Species:

  Mouse

• Overview:

  Purified recombinant protein of Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/57773

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9EP82

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/BC039272