Smad3 (NM_016769) Mouse Recombinant Protein

• Background:

  Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AU022421; Madh3

• Gene ID:

  17127

• UniProt:

  Q8BUN5

• Accession Number mRNA:

  NM_016769

• Chromosomal Location:

  9 C

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  48.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Smad3

• Species:

  Mouse

• Protein ID:

  NP_058049

• Overview:

  Purified recombinant protein of Mouse SMAD family member 3 (Smad3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/17127

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8BUN5

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016769

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_058049