Uba3 (NM_011666) Mouse Recombinant Protein

• Background:

  The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]

• Synonyms:

  A830034N06Rik; AI256736; AI848246; AW546539; Ube1c

• Gene ID:

  22200

• UniProt:

  Q8C878, Q3TL72

• Accession Number mRNA:

  NM_011666

• Chromosomal Location:

  6 D3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  49.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Uba3

• Species:

  Mouse

• Protein ID:

  NP_035796

• Overview:

  Purified recombinant protein of Mouse ubiquitin-like modifier activating enzyme 3 (Uba3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/22200

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8C878

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_011666

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_035796