Vps4b (NM_009190) Mouse Recombinant Protein

• Background:

  Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  8030489C12Rik; Skd1

• Gene ID:

  20479

• UniProt:

  P46467, Q3TN07, Q8BM73

• Accession Number mRNA:

  NM_009190

• Chromosomal Location:

  1 E2.1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  49.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Vps4b

• Species:

  Mouse

• Protein ID:

  NP_033216

• Overview:

  Purified recombinant protein of Mouse vacuolar protein sorting 4B (Vps4b), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/20479

• Uniprot URL:

  https://www.uniprot.org/uniprot/P46467

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_009190

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_033216