Fads2 (NM_019699) Mouse Recombinant Protein

• Background:

  Acts as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively (PubMed:9867867) . Subsequently, in the biosynthetic pathway of HUFA n-3 series, desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA) (22:6n-3), an important lipid for nervous system function (By similarity) . Desaturates palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum (By similarity) . Also desaturates (11E) -octadecenoate (trans-vaccenoate) (18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2900042M13Rik; Fadsd2

• Gene ID:

  56473

• UniProt:

  Q9Z0R9

• Accession Number mRNA:

  NM_019699

• Chromosomal Location:

  19 A

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  52.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Fads2

• Species:

  Mouse

• Protein ID:

  NP_062673

• Overview:

  Purified recombinant protein of Mouse fatty acid desaturase 2 (Fads2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/56473

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9Z0R9

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_019699

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_062673