Cpsf7 (NM_001164272) Mouse Recombinant Protein

• Background:

  Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) . Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF7 activates directly the mRNA 3'-processing machinery. Binds to pA signals in RNA substrates.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  5730453I16Rik; AL022757; C330017N18Rik

• Gene ID:

  269061

• UniProt:

  Q8BTV2

• Accession Number mRNA:

  NM_001164272

• Chromosomal Location:

  19 A

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  51.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Cpsf7

• Species:

  Mouse

• Protein ID:

  NP_001157744

• Overview:

  Purified recombinant protein of Mouse cleavage and polyadenylation specific factor 7 (Cpsf7), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/269061

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8BTV2

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001164272

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001157744