Sesn2 (NM_144907) Mouse Recombinant Protein

• Background:

  Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:18692468, PubMed:25259925) . This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1 (PubMed:24947615) . May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085) . May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed:18692468) . Has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity) . Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (By similarity) . However, this could not be confirmed (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  HI95; Ses2; SEST2

• Gene ID:

  230784

• UniProt:

  P58043

• Accession Number mRNA:

  NM_144907

• Chromosomal Location:

  4 D2.3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  54.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Sesn2

• Species:

  Mouse

• Protein ID:

  NP_659156

• Overview:

  Purified recombinant protein of Mouse sestrin 2 (Sesn2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/230784

• Uniprot URL:

  https://www.uniprot.org/uniprot/P58043

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_144907

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_659156