Gba (NM_008094) Mouse Recombinant Protein

• Background:

  Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:24211208) . Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698) . Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (By similarity) . Also plays a role in cholesterol metabolism (PubMed:24211208) . May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208) . The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (By similarity) . Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (By similarity) . Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  BetaGC; GBA1; GC; GCase; GLUC

• Gene ID:

  14466

• UniProt:

  P17439

• Accession Number mRNA:

  NM_008094

• Chromosomal Location:

  3 39.01 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  57.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Gba

• Species:

  Mouse

• Protein ID:

  NP_032120

• Overview:

  Purified recombinant protein of Mouse glucosidase, beta, acid (Gba), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/14466

• Uniprot URL:

  https://www.uniprot.org/uniprot/P17439

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008094

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032120