Racgap1 (BC010715) Mouse Recombinant Protein

• Background:

  Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Band25, GTPase, MgcRacGAP, gtl11

• Gene ID:

  26934

• UniProt:

  Q9WVM1

• Accession Number mRNA:

  BC010715

• Chromosomal Location:

  15 F1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  70.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Racgap1

• Species:

  Mouse

• Overview:

  Purified recombinant protein of Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/26934

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9WVM1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/BC010715