Vps53 (BC034371) Mouse Recombinant Protein

• Background:

  Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN) . The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  RP23-147P4.2

• Gene ID:

  68299

• UniProt:

  Q8CCB4

• Accession Number mRNA:

  BC034371

• Chromosomal Location:

  11 B5

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  73.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Vps53

• Species:

  Mouse

• Overview:

  Purified recombinant protein of Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/68299

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8CCB4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/BC034371