Ehhadh (NM_023737) Mouse Recombinant Protein

• Background:

  Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long straight chain fatty acids peroxisomal beta-oxidation pathway. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species. Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity) . With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (Probable) . Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (PubMed:24075987) . Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  1300002P22Rik; HD; L-PBE; LBFP; LBP; MFP; MFP1; PBFE

• Gene ID:

  74147

• UniProt:

  Q9DBM2

• Accession Number mRNA:

  NM_023737

• Chromosomal Location:

  16 B1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  78.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ehhadh

• Species:

  Mouse

• Protein ID:

  NP_076226

• Overview:

  Purified recombinant protein of Mouse enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/74147

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9DBM2

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_023737

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_076226