Chaf1a (NM_013733) Mouse Recombinant Protein

• Background:

  Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AL023013; AL024058; Cac1p; CAF-1

• Gene ID:

  27221

• UniProt:

  Q9QWF0

• Accession Number mRNA:

  NM_013733

• Chromosomal Location:

  17 D

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  101.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Chaf1a

• Species:

  Mouse

• Protein ID:

  NP_038761

• Overview:

  Purified recombinant protein of Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/27221

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9QWF0

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_013733

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_038761