Xrn2 (NM_011917) Mouse Recombinant Protein

• Background:

  Possesses 5'->3' exoribonuclease activity. May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites (By similarity) .[UniProtKB/Swiss-Prot Function]

• Gene ID:

  24128

• UniProt:

  Q9DBR1

• Accession Number mRNA:

  NM_011917

• Chromosomal Location:

  2 G2

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  108.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Xrn2

• Species:

  Mouse

• Protein ID:

  NP_036047

• Overview:

  Purified recombinant protein of Mouse 5'-3' exoribonuclease 2 (Xrn2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/24128

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9DBR1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_011917

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_036047