Supt5 (NM_013676) Mouse Recombinant Protein

• Background:

  Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AL033283; AL033321; AU019549; Spt5; Supt5h

• Gene ID:

  20924

• UniProt:

  O55201

• Accession Number mRNA:

  NM_013676

• Chromosomal Location:

  7 16.68 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  121.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Supt5

• Species:

  Mouse

• Protein ID:

  NP_038704

• Overview:

  Purified recombinant protein of Mouse suppressor of Ty 5 (Supt5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/20924

• Uniprot URL:

  https://www.uniprot.org/uniprot/O55201

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_013676

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_038704