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Nos3 (BC052636) Mouse Recombinant Protein

CAT: 0882-PM44732M5Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0882-PM44732M5Size:20 µg
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Background
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
ENOS, ecNOS
Gene ID
18127
UniProt
P70313
Accession Number mRNA
BC052636
Chromosomal Location
5 11.32 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
146 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Nos3
Species
Mouse
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/18127
Uniprot URL
https://www.uniprot.org/uniprot/P70313
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/BC052636

UniProtKB · P70313

Nitric oxide synthase 3

NOS3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P70313
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Nos3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
1.14.13.39
Processing
—
Secondary accessions
O55056, Q7TSV7
Protein keywords

Ligand

CalciumFADFlavoproteinFMNHemeIronMetal-bindingNADPZinc

Molecular function

Calmodulin-bindingOxidoreductase

Cellular component

Cell membraneCytoplasmCytoskeletonGolgi apparatusMembrane

PTM

LipoproteinMyristatePalmitatePhosphoprotein

Technical term

Reference proteome

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