Mxra8 (NM_024263) Mouse Recombinant Protein

• Background:

  Transmembrane protein which can modulate activity of various signaling pathways, probably via binding to integrin ITGAV:ITGB3 (PubMed:18366072, PubMed:22492581, PubMed:29702220) . Mediates heterophilic cell-cell interactions in vitro (PubMed:18366072) . Inhibits osteoclastogenesis downstream of TNFSF11/RANKL and CSF1, where it may function by attenuating signaling via integrin ITGB3 and MAP kinase p38 (PubMed:22492581) . Plays a role in cartilage formation where it promotes proliferation and maturation of growth plate chondrocytes (PubMed:29702220) . Stimulates formation of primary cilia in chondrocytes (PubMed:29702220) . Enhances expression of genes involved in the hedgehog signaling pathway in chondrocytes, including the hedgehog signaling molecule IHH; may also promote signaling via the PTHLH/PTHrP pathway (PubMed:29702220) . Plays a role in angiogenesis where it suppresses migration of endothelial cells and also promotes their apoptosis (By similarity) . Inhibits VEGF-induced activation of AKT and p38 MAP kinase in endothelial cells (By similarity) . Also inhibits VTN (vitronectin) -mediated integrin ITGAV:ITGB3 signaling and activation of PTK2/FAK (By similarity) . May play a role in the maturation and maintenance of the blood-brain barrier (PubMed:14603461) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  1200013A08Rik; 1700095D18Rik; AI131686; Asp3; Dicam

• Gene ID:

  74761

• UniProt:

  Q9DBV4

• Accession Number mRNA:

  NM_024263

• Chromosomal Location:

  4 87.58 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  50.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Mxra8

• Species:

  Mouse

• Protein ID:

  NP_077225

• Overview:

  Purified recombinant protein of Mouse matrix-remodelling associated 8 (Mxra8), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/74761

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9DBV4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_024263

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_077225